Society of Toxicology Annual Meeting: Repeatability of a Depth of Injury Test Method for the Classification of Eye Irritants

The in vitro depth of injury (DoI) test method classifies eye irritants by measuring the penetration depth of a test substance into a surrogate corneal substrate, generating a classification that maps onto GHS Category 1, Category 2, and No Category endpoints. For regulatory acceptance, test methods must demonstrate adequate repeatability, defined as the ability to produce consistent results across independent test runs within the same laboratory. This abstract evaluates the within-laboratory repeatability of the DoI method using a panel of reference chemicals spanning the full range of GHS eye irritation categories. Coefficient of variation (CV) and classification concordance were calculated across multiple independent test runs. Results demonstrated low variability in DoI measurements and high classification concordance across runs, supporting the method's suitability for regulatory use. The data contribute to the prevalidation package for the IVD EIT and support its development toward OECD test guideline status.